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| Abstract : Universiti Pendidikan Sultan Idris |
| In order to achieve phytoremediation during agricultural production, it is essential to identify high genotypes yield that is able of accumulating many types of heavy metals not in the edible parts which have significant nutritional value but in the non-edible parts. This study conducted to estimate the heavy metal ATPases 5 (HMA5), Phytochelatins synthase (PCs) and metallothionein 2 (MT2) genes expression in plant Vicia faba in response to an elevated concentration of copper and zinc in nutrient media. Using Quantitative Real-Time PCR (RT-qPCR) technique, the results of hydroponic culture methods with high concentrations of copper (75 �Mol / L) and high concentration of zinc (500 � Mol / L) showed high expression level for the three genes of Vicia faba plant compared with control. Established that the expression of the genes under the influence of copper ion was higher than the expression under zinc ion influence. Besides that, gene expression increased with increased exposure time to zinc ion, also in the case of copper ion exposure time, all genes expression slightly increases with increased exposure time. In response to excess copper and zinc, an increase in the expression of genes (HMA5, PCs and MT2) involved in plant protection, providing the possibility of its transfer from the cytosol to the apoplast demonstrate that this plant might be useful for phytoremediation of moderately polluted areas with copper or zinc. ? 2021, Institute of Medico-Legal Publications. All rights reserved. |
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